Nucleosil NH2, 100Å, 10µm, 4.6 x 250mm, ea.
Method: HPLC, analytical
Brand: NUCLEOSIL
Base material: NUCLEOSIL – the standard silica
Phase: NUCLEOSIL NH2 100-10
Surface chemistry: Aminopropyl (NH2) modification
Mode: Multi-mode (NP and RP), Normal phase (NP)
Type: EC HPLC column (analytical)
Hardware: Stainless steel
Column length: 250 mm
Column inner diameter: 4.6 mm
Particle type: Fully porous particles (FPP)
Particle shape: Spherical
Particle size: 10 µm
Eluent in HPLC column: n-heptane
Recommended application(s): HPLC, Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases)
Endcapped: No
Carbon content: 3.5 %
pH stability: 2.0–8.0
Pore size: 100 Å
Specific surface according to BET1: 350 m²/g
Temperature stability (max): 60 °C
Pressure stability (max): 400 bar / 5800 psi
Column volume: 4.15 mL
Storage temperature: 15–25 °C / 59–77 °F
Scope of delivery: 1x analytical HPLC column, instruction leaflet, certificate of analysis
Gross weight (incl. packaging): 271.1 g / 0.6 lbs
Packaging dimensions: 115 x 400 x 50 mm / 4.53 x 15.75 x 1.97 Inch
Hazardous material: No
USP listing: L8
- Descripción
Descripción
SKU: 720025.46
Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions. These HPLC columns are packed with polar aminopropyl modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
